File Name: C:\Program Files\Bio-Rad\Bio-Plex Manager 5.0\users\Craig Miller\Results\Saliva IgG Luitpold d57 070113.rbx
Analyte: IgG (77)
Acquisition Date: 01-Jul-2013, 01:41 PM
Reader Serial Number: LX10008241401
Plate ID: 
RP1 PMT (Volts): 647.13
RP1 Target: 17026
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgG (77) B A1,B1,C1,D1 0 117.4 117.4 3.35 1.68 2.85
IgG (77) S1 E1,F1 2 Standard 1 19884.5 19767.1 0 0 0 968.28 1000 97 968.28
IgG (77) S2 G1,H1 0 Standard 2 17706.8 17589.4 22.27 15.75 0.13 506.52 500 101 506.52
IgG (77) S3 A2,B2 0 Standard 3 15072 14954.6 340.83 241 2.26 266.58 250 107 266.58
IgG (77) S4 C2,D2 0 Standard 4 11228.5 11111.1 111.02 78.5 0.99 117.22 125 94 117.22
IgG (77) S5 E2,F2 0 Standard 5 8326.5 8209.1 20.51 14.5 0.25 63.46 62.5 102 63.46
IgG (77) S6 G2,H2 0 Standard 6 5410 5292.6 12.73 9 0.24 31.67 31.25 101 31.67
IgG (77) S7 A3,B3 0 Standard 7 3130 3012.6 18.38 13 0.59 15.49 15.63 99 15.49
IgG (77) S8 C3,D3 2 Standard 8 1706 1588.6 0 0 0 7.83 7.81 100 7.83
IgG (77) X1 E3,F3 0 4661 370.3 252.9 8.84 6.25 2.39 *1562.63 ***
IgG (77) X2 G3,H3 0 4331 1369.5 1252.1 120.92 85.5 8.83 *6207.83 ***
IgG (77) X3 A4,B4 0 4326 1417 1299.6 4.24 3 0.3 *6432.83 ***
IgG (77) X4 C4,D4 0 4651 1966.8 1849.4 39.95 28.25 2.03 9122.88 9122.88
IgG (77) X5 E4,F4 0 4653 1163.3 1045.9 3.89 2.75 0.33 *5241.82 ***
IgG (77) X6 G4,H4 0 4654 3723 3605.6 60.81 43 1.63 19165.16 19165.16
IgG (77) X7 A5,B5 0 4655 1477.5 1360.1 0.71 0.5 0.05 *6720.93 ***
IgG (77) X8 C5,D5 0 4657 764.5 647.1 144.96 102.5 18.96 *3407.79 ***
IgG (77) X9 E5,F5 0 4658 404 286.6 9.9 7 2.45 *1727.22 ***
IgG (77) X10 G5,H5 0 4659 701.8 584.4 16.62 11.75 2.37 *3120.01 ***
IgG (77) X11 A6,B6 0 4662 6372.3 6254.9 148.14 104.75 2.32 40471.09 40471.09
IgG (77) X12 C6,D6 0 4663 654 536.6 19.8 14 3.03 *2900.44 ***
IgG (77) X13 E6,F6 0 4665 434.5 317.1 9.19 6.5 2.12 *1874.06 ***
IgG (77) X14 G6,H6 0 4666 601 483.6 7.07 5 1.18 *2655.76 ***
IgG (77) X15 A7,B7 0 4667 4044 3926.6 79.9 56.5 1.98 21297.54 21297.54
IgG (77) X16 C7,D7 0 4669 3752.5 3635.1 4.95 3.5 0.13 19356.81 19356.81
IgG (77) X17 E7,F7 0 4670 1801 1683.6 9.9 7 0.55 8293.7 8293.7
IgG (77) X18 G7,H7 0 4671 1405.8 1288.4 6.72 4.75 0.48 *6379.45 ***
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgG (77) B A1 0 116.5 116.5 374
IgG (77) B B1 0 113 113 293
IgG (77) B C1 0 120 120 365
IgG (77) B D1 0 120 120 330
IgG (77) S1 E1 0 19884.5 19767.1 968.28 1000 97 968.28 254
IgG (77) S1 F1 1 19924 19806.6 1000 265
IgG (77) S2 G1 0 17691 17573.6 504.4 500 101 504.4 299
IgG (77) S2 H1 0 17722.5 17605.1 508.66 500 102 508.66 298
IgG (77) S3 A2 0 15313 15195.6 281.58 250 113 281.58 283
IgG (77) S3 B2 0 14831 14713.6 252.52 250 101 252.52 309
IgG (77) S4 C2 0 11307 11189.6 119.15 125 95 119.15 253
IgG (77) S4 D2 0 11150 11032.6 115.32 125 92 115.32 305
IgG (77) S5 E2 0 8341 8223.6 63.66 62.5 102 63.66 305
IgG (77) S5 F2 0 8312 8194.6 63.26 62.5 101 63.26 307
IgG (77) S6 G2 0 5401 5283.6 31.59 31.25 101 31.59 276
IgG (77) S6 H2 0 5419 5301.6 31.74 31.25 102 31.74 320
IgG (77) S7 A3 0 3143 3025.6 15.56 15.63 100 15.56 326
IgG (77) S7 B3 0 3117 2999.6 15.41 15.63 99 15.41 301
IgG (77) S8 C3 1 1655 1537.6 7.81 289
IgG (77) S8 D3 0 1706 1588.6 7.83 7.81 100 7.83 238
IgG (77) X1 E3 0 376.5 259.1 *1593.30 *** 270
IgG (77) X1 F3 0 364 246.6 *1531.87 *** 259
IgG (77) X2 G3 0 1284 1166.6 *5805.32 *** 293
IgG (77) X2 H3 0 1455 1337.6 *6613.58 *** 321
IgG (77) X3 A4 0 1420 1302.6 *6447.07 *** 315
IgG (77) X3 B4 0 1414 1296.6 *6418.59 *** 328
IgG (77) X4 C4 0 1938.5 1821.1 8980.36 8980.36 310
IgG (77) X4 D4 0 1995 1877.6 9265.91 9265.91 306
IgG (77) X5 E4 0 1160.5 1043.1 *5229.04 *** 310
IgG (77) X5 F4 0 1166 1048.6 *5254.59 *** 276
IgG (77) X6 G4 0 3766 3648.6 19444.79 19444.79 238
IgG (77) X6 H4 0 3680 3562.6 18887.35 18887.35 258
IgG (77) X7 A5 0 1478 1360.6 *6723.32 *** 309
IgG (77) X7 B5 0 1477 1359.6 *6718.55 *** 337
IgG (77) X8 C5 0 867 749.6 *3877.22 *** 331
IgG (77) X8 D5 0 662 544.6 *2937.27 *** 261
IgG (77) X9 E5 0 411 293.6 *1761.06 *** 281
IgG (77) X9 F5 0 397 279.6 *1693.28 *** 301
IgG (77) X10 G5 0 690 572.6 *3066.03 *** 303
IgG (77) X10 H5 0 713.5 596.1 *3173.94 *** 330
IgG (77) X11 A6 0 6267.5 6150.1 39443.25 39443.25 292
IgG (77) X11 B6 0 6477 6359.6 41517.13 41517.13 282
IgG (77) X12 C6 0 640 522.6 *2835.92 *** 259
IgG (77) X12 D6 0 668 550.6 *2964.88 *** 261
IgG (77) X13 E6 0 441 323.6 *1905.15 *** 310
IgG (77) X13 F6 0 428 310.6 *1842.89 *** 255
IgG (77) X14 G6 0 606 488.6 *2678.90 *** 257
IgG (77) X14 H6 0 596 478.6 *2632.61 *** 313
IgG (77) X15 A7 0 3987.5 3870.1 20914.59 20914.59 194
IgG (77) X15 B7 0 4100.5 3983.1 21683.84 21683.84 236
IgG (77) X16 C7 0 3749 3631.6 19334.02 19334.02 257
IgG (77) X16 D7 0 3756 3638.6 19379.6 19379.6 314
IgG (77) X17 E7 0 1794 1676.6 8259.05 8259.05 250
IgG (77) X17 F7 0 1808 1690.6 8328.38 8328.38 264
IgG (77) X18 G7 0 1401 1283.6 *6356.92 *** 312
IgG (77) X18 H7 0 1410.5 1293.1 *6401.98 *** 288
Sampling Errors: 1 - Low bead #, 2 - Agg beads, 3 - Classify %, 4 - Region selection, 5 - Platform temperature
***Value not available / --- = Designated as an outlier
*Value extrapolated beyond standard range
OOR = Out of Range / OOR> = Out of Range Above  / OOR< = Out of Range Below
Exp Conc = Expected Concentration  /  Obs Conc = Observed  Concentration
Conc in Range = Unknown sample concentrations within range where standards recovery is 70-130%
Regression Type: Logistic - 5PL
Std. Curve: FI = -2.99313 + (25546.5 + 2.99313) / ((1 + (Conc / 29.7439)^-0.618294))^2.33503
FitProb. = 0.8771, ResVar. = 0.2277